The Missile Launcher is a weapon exclusive to Duke Nukem 64 that replaces the Devastator. Since the RPG was replaced with the Grenade Launcher, the Missile Launcher is the weapon which carries the missiles. It is almost identical to the RPG, although sightly slower (the same fire rate as the Shotgun). The player can carry as much ammunition as a RPG, 50, getting 5 missiles per item.

It is first found on the level The Abyss.

Using the Heat Seek Missiles sub ammunition, the player can fire missiles which traces enemies. It has the same damage as the normal missile, but can easily take down an enemy such as a RPV which usually is hard to be hit by a missile. The maximum amount of missiles is 25, half of the default ammunition.